Directed-migration of fibroblasts using the opto-CXCR4 system. Cells migrate following a gradient of light, the centre of the light source is depicted (+). Untransfected cells (not mCherry positive) do not migrate and light has no effect on them.
Directed migration of fibroblasts (red), to the direction of light (circle with cross). While fibroblasts migrate they interact with other cells (green) via an engineered receptor system that can be controlled by a small compound.
Measuring the directed-migration of fibroblasts (images are frames of the video above)
HeLa cells induced to migrate using the LOV-Rac1 system.
Organoid culture (MDA-MB-231 breast cancer cells) with labelled E-cadherin, Draq5 and Phalloidin.
PC3 (PROSTATE CANCER CELLS)
MDA-MB-231 (BREAST CANCER CELLS)
The principle of the CANDELS assay (from our article: Trehan A, et al. Cell Commun Signal. 2014; 12: 70):
and the resulting luminescence readout:
This assay has been used to determine the functionality of patient-derived mutant gonadotropin receptors (Rivero-Müller A, et al. Eur J Endocrinol. 2015 Jun;172(6):K27-36. doi: 10.1530/EJE-14-1095) and the luteinising hormone beta subunit (LHB) (Potorac I, et al. J Endocrinol. 2016 Dec;231(3):197-207), as well as the interactions between ligand-loaded nanoparticles to the trageting receptor (from our work Paramonov V, et al. Nanotheranostics 2018; 2(4):320-346. doi:10.7150/ntno.23826):
The principle of the EMT/MET sensor cells (from our article: Kiełbus et al. (2019) Cells 8(12), 1644)
The fluorescence of mCherry goes hand-in-hand with the endogenous expression of Vimentin
Video abstract of Mung et al Cell Comm and Signaling (2021) can be seen here (link)
Video abtract of Kałafut et al (2022) optogenetic control of NOTCH1 signalling. Cell Communication and Signaling, can be found here (link)
3D modelling of the LHB/CGA heterodimer. From: Potorac I*, Rivero-Muller A*, Trehan A*, Kielbus M, Jozwiak K, Pralong F, Hafidi A, Thiry A, Menage J-J, Huhtaniemi IT, Beckers A, and Daly AF (2016) A vital region for human glycoprotein hormone trafficking revealed by an LHB mutation. J Endocrinol 231(3):197-207, doi:10.1530/JOE-16-0384. (http://joe.endocrinology-journals.org/content/233/1.cover-expansion) (https://www.ncbi.nlm.nih.gov/pubmed/27656125).
Outline of the ALFIRE procedure. From: Rivero-Muller A, Lajić S, Huhtaniemi I. (2007) Assisted Large Fragment Insertion by Red/ET-recombination (AL-FIRE) - An Alternative and Enhanced Method for Large Fragment Recombineering. Nucleic Acids Research 35(10):e78. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904275/).
Targeting the somatostatin receptors using nanoparticles. From: Paramonov VM, Desai D, Kettiger H, Mamaeva V, Rosenholm JM, Sahlgren C, Rivero-Müller A. Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?. Nanotheranostics 2018; 2(4):320-346. doi:10.7150/ntno.23826. Available from http://www.ntno.org/v02p0320.htm
Molecular architecture of the carapace of a decorator crab. From: Sanka I, Suyono EA, Rivero-Müller A, and Alam P (2016) Carapace surface architectures facilitate camouflage of the decorator crab Tiarinia cornigera. Acta Biomaterialia S1742-7061(16)30239-2. doi: 10.1016/j.actbio.2016.05.021. https://www.ncbi.nlm.nih.gov/pubmed/27184402/
E.coli expressing different fluorescent proteins.
